is a medicinal plant used in traditional medicine. The pharmacological activities of any plant is because of the presence of primary metabolites, secondary metabolites and its secretary products, comprising the phenolic compounds, alkaloids, tannins, saponins, carbohydrates, glycosides, flavanoids, steroids, etc. Many infections can be treated by phytochemicals possessing potent antibacterial efficiency. The increasing antibiotic resistance exhibited by microorganisms has led to the phytochemical screening of medicinal plants for antimicrobial activity. Piperine will start crystallizing.Herbal medicines are used for the treatment of many infectious diseases throughout the history of mankind. Now, perform vacuum filtration and take out as much paste as you can.Īdd a small amount of isopropyl alcohol and allow it to stand for a few hours or days. So, now we are going to add water and immediately, the piperine starts coming out and the mixture gets cloudy. Remember that piperine does not react with KOH and it also hates water. This will make all the impurities water-soluble. The acids, fats that were extracted along with piperine will react with KOH. Now add this KOH mixture to our previous solvent mixture. For that, add 1g of KOH to 10 ml of isopropyl alcohol. Perform simple distillation to get rid of most of the isopropyl alcohol. To separate these things, first, start with isopropyl alcohol. Now start the extraction process as mentioned previously.Īt the end of the procedure, we will be left with isopropyl alcohol + peperine + other isopropyl alcohol-soluble components. But remember that the powder consistency should be granular and not too fine. Put the black pepper powder inside the thimble. So, we use isopropyl alcohol as a solvent.Īs mentioned in the working of the soxhlet extractor, we set up the assembly. We will see how the extraction process works for a better understanding of Soxhlet extraction.īlack pepper contains piperine which is soluble in isopropyl alcohol. We use the soxhlet extractor for a variety of applications, one of which is piperine extraction from black pepper.
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